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pAct-FRT-stop-FRT3-FRT-FRT3-Gal4 attB
(Plasmid #52889)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 52889 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAct5-FRT-stop-FRT-SV40polyA attB
  • Backbone manufacturer
    David Strutt (Brittle et al., 2010)
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Gal4
  • Species
    S. cerevisiae (budding yeast)
  • Entrez Gene
    GAL4 (a.k.a. YPL248C, GAL81)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAct-FRT-stop-FRT3-FRT-FRT3-Gal4 attB was a gift from Iswar Hariharan (Addgene plasmid # 52889 ; http://n2t.net/addgene:52889 ; RRID:Addgene_52889)

  • For your References section:

    CoinFLP: a system for efficient mosaic screening and for visualizing clonal boundaries in Drosophila. Bosch JA, Tran NH, Hariharan IK. Development. 2015 Feb 1;142(3):597-606. doi: 10.1242/dev.114603. 10.1242/dev.114603 PubMed 25605786
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