FLAG-HA-pcDNA3.1-
(Plasmid #52535)

• Purpose: (Empty Backbone) enables eukaryotic expression of N-terminally FLAG-HA-tagged fusion proteins
• Depositing Lab: Adam Antebi
• Publication: Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14)00069-0. doi: 10.1016/j.devcel.2014.01.028.

Backbone

• Vector backbone: pcDNA3.1-
• Backbone manufacturer: Invitrogen
• Backbone size (bp): 5501
• Modifications to backbone: addded/inserted sequences encoding "FLAG–5xGly–HA–5xGly" tag at start of MCS to allow for fusion protein production
• Vector type: Mammalian Expression
• Promoter: CMV
• Selectable markers: Neomycin (select with G418)
• Tag / Fusion Protein:
  • FLAG-HA (N terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: T7
• 3′ sequencing primer: BGH poly reverse

Resource Information

• A portion of this plasmid was derived from a plasmid made by:
• Articles Citing this Plasmid:
  • 45 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Information for Cloning Grade DNA (Catalog # 52535-DNA.cg) ( Back to top)

Purpose

Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.

Delivery

• Amount: 2 µg
• Guaranteed Concentration: 100 ng/µl +/- 5 ng/µl
• Pricing: $105 USD
• Storage: DNA can be stored at 4℃ (short term) or -20℃ (long term).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Addgene has verified this plasmid using Next Generation Sequencing. Results are available here