RGS-6xHis-pcDNA3.1-
(Plasmid #52534)

• Purpose: (Empty Backbone) enables eukaryotic expression of N-terminally RGS-6xHis–tagged fusion proteins
• Depositing Lab: Adam Antebi
• Publication: Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14)00069-0. doi: 10.1016/j.devcel.2014.01.028.

Backbone

• Vector backbone: pcDNA3.1-
• Backbone manufacturer: Invitrogen
• Backbone size (bp): 5454
• Modifications to backbone: addded/inserted sequence encoding "RGS-6xHis" tag at start of MCS to allow for fusion protein production
• Vector type: Mammalian Expression
• Promoter: CMV
• Selectable markers: Neomycin (select with G418)
• Tag / Fusion Protein:
  • RGS-6xHis (N terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: T7
• 3′ sequencing primer: BGH poly reverse

Resource Information

• A portion of this plasmid was derived from a plasmid made by:
• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry