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pAAV-synP-FLEX-splitTVA-EGFP
(Plasmid #52474)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 52474 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 4436
  • Total vector size (bp) 5690
  • Vector type
    Mammalian Expression, AAV, Cre/Lox

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Growth instructions
    Stbl3 at 37C
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    splitTVA-P2A-EGFP
  • Alt name
    sTpE
  • Species
    Synthetic
  • Insert Size (bp)
    1254
  • Promoter Synapsin-1

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer CTGCGTATGAGTGCAAG TTGTACAGCTCGTCCATG
  • 3′ sequencing primer cagcgtatccacatagcg
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Alternative plasmid name: pAAV-synP-FLEX-sTpE

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-synP-FLEX-splitTVA-EGFP was a gift from Ian Wickersham (Addgene plasmid # 52474 ; http://n2t.net/addgene:52474 ; RRID:Addgene_52474)

  • For your References section:

    Cell type-specific genetic and optogenetic tools reveal hippocampal CA2 circuits. Kohara K, Pignatelli M, Rivest AJ, Jung HY, Kitamura T, Suh J, Frank D, Kajikawa K, Mise N, Obata Y, Wickersham IR, Tonegawa S. Nat Neurosci. 2014 Feb;17(2):269-79. doi: 10.1038/nn.3614. Epub 2013 Dec 15. 10.1038/nn.3614 PubMed 24336151
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