pAAV-synP-FLEX-splitTVA-EGFP
(Plasmid #52474)

• Purpose: Expresses splitTVA-EGFP
• Depositing Lab: Ian Wickersham
• Publication: Kohara et al Nat Neurosci. 2014 Feb;17(2):269-79. doi: 10.1038/nn.3614. Epub 2013 Dec 15.

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Backbone

• Vector backbone: pAAV
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 4436
• Total vector size (bp): 5690
• Vector type: Mammalian Expression, AAV, Cre/Lox

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Growth instructions: Stbl3 at 37C
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: splitTVA-P2A-EGFP
• Alt name: sTpE
• Species: Synthetic
• Insert Size (bp): 1254
• Promoter: Synapsin-1

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CTGCGTATGAGTGCAAG TTGTACAGCTCGTCCATG
• 3′ sequencing primer: cagcgtatccacatagcg

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Alternative plasmid name: pAAV-synP-FLEX-sTpE