pFGL815
(Plasmid
#52322)
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Purpose(Empty Backbone) A vector backbone for ATMT (Agrobacterium tumerfaciens-mediated transformation). There is a 69-bp MCS (multiple cloning site) which contains 12 unique restriction sites.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 52322 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCAMBIA1300
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Modifications to backbone1. The CaMV_35S promoter and a hygromycin-resistance cassette have been removed in between the LB and RB. 2. A Sph I restriction site after the RB has been eliminated, resulting a unique Sph I site within the MCS.
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Vector typeBacterial Expression ; Agrobacterium
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)XL1 Blue
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFGL815 was a gift from Naweed Naqvi (Addgene plasmid # 52322 ; http://n2t.net/addgene:52322 ; RRID:Addgene_52322) -
For your References section:
Sulfonylurea resistance reconstitution as a novel strategy for ILV2-specific integration in Magnaporthe oryzae. Yang F, Naqvi NI. Fungal Genet Biol. 2014 Apr 24. pii: S1087-1845(14)00059-0. doi: 10.1016/j.fgb.2014.04.005. 10.1016/j.fgb.2014.04.005 PubMed 24769367
Map uploaded by the depositor.
