pRmHA3-CH2F2-CG9866
(Plasmid
#52286)
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PurposeExpresses Drosophila Ataxin-7
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 52286 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepRmHA3
- Backbone size w/o insert (bp) 3991
- Total vector size (bp) 6910
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameAtaxin-7
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Alt nameCG9866
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SpeciesD. melanogaster (fly)
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Insert Size (bp)2919
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Entrez GeneCG9866 (a.k.a. Dmel_CG9866, Dmel\CG9866)
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Tag
/ Fusion Protein
- 2xHA 2xFLAG (C terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRmHA3-CH2F2-CG9866 was a gift from Jerry Workman (Addgene plasmid # 52286 ; http://n2t.net/addgene:52286 ; RRID:Addgene_52286) -
For your References section:
Loss of Drosophila Ataxin-7, a SAGA subunit, reduces H2B ubiquitination and leads to neural and retinal degeneration. Mohan RD, Dialynas G, Weake VM, Liu J, Martin-Brown S, Florens L, Washburn MP, Workman JL, Abmayr SM. Genes Dev. 2014 Feb 1;28(3):259-72. doi: 10.1101/gad.225151.113. 10.1101/gad.225151.113 PubMed 24493646
Map uploaded by the depositor.
