pSG5-ERRbeta-delta10
(Plasmid #52187)

• Purpose: Expresses human ERRbeta-delta10 splice variant in mammalian cells
• Depositing Lab: Rebecca Riggins
• Publication: Heckler et al Cell Cycle. 2014 Dec 12:0.

Backbone

• Vector backbone: pSG5
• Backbone manufacturer: Agilent
• Backbone size w/o insert (bp): 4100
• Total vector size (bp): 5639
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Estrogen-related receptor beta
• Species: H. sapiens (human)
• Insert Size (bp): 1539
• Mutation: wild type sequence; codon optimized to exclude EcoRI and BamHI sites; made shRNA resistant
• Entrez Gene: ESRRB (a.k.a. DFNB35, ERR beta-2, ERR2, ERRb, ERRbeta2, ESRL2, NR3B2)
• Promoter: SV40

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: SV40pA-R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Synthesized by Genewiz
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry