pChlr-pNEB193
(Plasmid #52136)

• Purpose: (Empty Backbone) A backbone plasmid for creation of additional PUF repeat modules
• Depositing Lab: Huimin Zhao
• Publication: Abil et al J Biol Eng. 2014 Mar 1;8(1):7.

Backbone

• Vector backbone: pNEB193
• Backbone manufacturer: New England Biolabs
• Backbone size (bp): 2280
• Modifications to backbone: Replaced the original ampicillin resistance gene Bla with the chloramphenicol resistance gene Cam from pACYC (New England Biolabs) using the Gibson Assembly method.
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: High Copy

Cloning Information

• 5′ sequencing primer: M13 For
• 3′ sequencing primer: M13 Rev

Resource Information

• Supplemental Documents:
  • Annotated GenBank file (updated Feb 2017)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that the plasmid contains a 1.3 kb insert containing Transposon 10 between bp# 51-52 of the original plasmid sequence. This insertion was not intentional, but does not affect the use of the plasmid to generate additional PUF repeat modules. Alternatively, any other PUF repeat plasmid can be similarly used to create new PUF repeate modules, since they all have the necessary SacI and HindIII sites.