9X NFAT-apha-MHC-Luc
(Plasmid #51941)

• Purpose: 9 copies of NFAT sites in minimal alphaMHC-pGL-3
• Depositing Lab: Jeffery Molkentin
• Publication: Wilkins et al Circ Res. 2004 Jan 9;94(1):110-8. Epub 2003 Dec 1.

Backbone

• Vector backbone: pGL-3 Basic
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 4818
• Total vector size (bp): 5100
• Vector type: Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: 9x NFAT binding site
• Alt name: Nine copies of an NFAT binding site from the IL-4 promoter (5′-TGGAAAATT-3′)
• Insert Size (bp): 81

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: RVprimer3
• 3′ sequencing primer: na

Gene/Insert 2

• Gene/Insert name: alpha-MHC
• Alt name: minimal promoter from the α-myosin heavy chain gene (−164 to +16)
• Insert Size (bp): 180
• Tag / Fusion Protein:
  • luciferase (C terminal on backbone)

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: na
• 3′ sequencing primer: LucNRev

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry