pcDNA1-GATA4-opposite orientation
(Plasmid
#51928)
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Purposefor T7 directed antisense production of mouse GATA4
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 51928 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA1
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 4000
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameGATA4 reverse orientation
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Alt nameGATA binding protein 4
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SpeciesM. musculus (mouse)
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Insert Size (bp)1700
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Entrez GeneGata4 (a.k.a. Gata-4)
- Promoter CMV
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
1.7 kb mouse GATA4 EcoRI fragment, 80 bp of 5'UTR
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA1-GATA4-opposite orientation was a gift from Jeffery Molkentin (Addgene plasmid # 51928 ; http://n2t.net/addgene:51928 ; RRID:Addgene_51928) -
For your References section:
The transcription factors GATA4 and GATA6 regulate cardiomyocyte hypertrophy in vitro and in vivo. Liang Q, De Windt LJ, Witt SA, Kimball TR, Markham BE, Molkentin JD. J Biol Chem. 2001 Aug 10;276(32):30245-53. Epub 2001 May 16. 10.1074/jbc.M102174200 PubMed 11356841