pcDNA3-Xt-VSP
(Plasmid #51887)

• Purpose: expression of Xt-VSP in Xenopus oocytes or mammalian cells
• Depositing Lab: Laurinda Jaffe
• Publication: Ratzan et al J Cell Physiol. 2011 Nov;226(11):2740-6. doi: 10.1002/jcp.22854.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Vector type: Mammalian Expression ; Xenopus oocyte expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: VSP
• Species: X. tropicalis
• Mutation: see comments*
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: EcoRV (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH Reverse

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Plasmid encoding Xt-VSP (NM_001015951.2, clone # 77E02) was purchased from GeneService (Cambridge, UK).

*There is a Y242H aa residue substitution compared to the listed genbank ref seq. This amino acid difference is believed to be a polymorphism that does not affect function.