pSEZ-1
(Plasmid #51868)

• Purpose: Expression of cpYFP with a mitochondrial-targeting sequence (mt-cpYFP) in C. elegans
• Depositing Lab: Meng-Qiu Dong
• Publication: Shen et al Nature. 2014 Feb 12. doi: 10.1038/nature13012.

Backbone

• Vector backbone: pSDH2(P-2)GFP
• Backbone manufacturer: B. D. Lemire
• Backbone size w/o insert (bp): 3470
• Modifications to backbone: pSDH2(P-2)GFP contains the promoter and the mitochondrial localization sequence of SDHB-1 inserted into the vector pPD95.79. pSEZ-1 replaces the GFP with the cpYFP coding sequence amplified by PCR from pShuttle–CMV-mito-cpYFP.
• Vector type: Worm Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: psdhb-1::mtLS-cpYFP
• Species: C. elegans (nematode)
• Insert Size (bp): 178
• Promoter: sdhb-1

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: M13-Rev
• 3′ sequencing primer: EGFP-N

Resource Information

• Supplemental Documents:
  • sequence doc
• A portion of this plasmid was derived from a plasmid made by: cpYFP coding region was a gift from He-Ping Cheng (Peking University); the backbone vector was a gift from B.D. Lemire (University of Alberta)
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry