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pENTR-AtMIR390a-B/c
(Plasmid #51778)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 51778 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pENTR
  • Backbone size w/o insert (bp) 2580
  • Total vector size (bp) 4491
  • Vector type
    GATEWAY entry vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Kanamycin, 25 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DB3.1
  • Growth instructions
    E. coli DB3.1 (or other strain resistant to ccdB) at 37C
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AtMIR390a-B/c
  • Species
    A. thaliana (mustard weed)
  • Insert Size (bp)
    1911
  • Mutation
    A. thaliana MIR390a precursor sequence including a chloramphenicol-ccdB cassette flanked by two inverted BsaI sites. The BsaI site in the ccdB gene was mutated to block the site.

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pENTR-AtMIR390a-B/c was a gift from James Carrington (Addgene plasmid # 51778 ; http://n2t.net/addgene:51778 ; RRID:Addgene_51778)

  • For your References section:

    New Generation of Artificial MicroRNA and Synthetic Trans-Acting Small Interfering RNA Vectors for Efficient Gene Silencing in Arabidopsis. Carbonell A, Takeda A, Fahlgren N, Johnson SC, Cuperus JT, Carrington JC. Plant Physiol. 2014 Mar 19. 10.1104/pp.113.234989 PubMed 24647477
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