pFK210B-AtMIR390a-B/c
(Plasmid #51777)

• Purpose: Plant expression vector (35S) for direct cloning of artificial microRNAs into Arabidopsis thaliana MIR390a precursor
• Depositing Lab: James Carrington
• Publication: Carbonell et al Plant Physiol. 2014 Mar 19.

Backbone

• Vector backbone: pGreen III
• Backbone size w/o insert (bp): 6005
• Total vector size (bp): 7916
• Modifications to backbone: A BsaI site of the backbone ws mutated
• Vector type: Plant Expression
• Selectable markers: Basta

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Spectinomycin, 25 & 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DB3.1
• Growth instructions: E. coli DB3.1 (or other strain resistant to ccdB) at 37C
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: AtMIR390a-B/c
• Species: A. thaliana (mustard weed)
• Insert Size (bp): 1911
• Mutation: A. thaliana MIR390a precursor sequence including a chloramphenicol-ccdB cassette flanked by two inverted BsaI sites. The BsaI site in the ccdB gene was mutated to block the site.

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: M13-F20
• 3′ sequencing primer: M13-Rev

Resource Information

• Supplemental Documents:
  • Annotated sequence pFK210B-AtMIR390a-B/c
  • genbank
  • cloning protocol
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry