pLSLGUS
(Plasmid #51517)

• Purpose: pLSL with GUS coding sequence followed by 3 kb of filler sequence Rep is not included on this plasmid
• Depositing Lab: Daniel Voytas
• Publication: Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi: 10.1105/tpc.113.119792

Backbone

• Vector backbone: pCAMBIA1300
• Modifications to backbone: cis-acting replicational elements from the bean yellow dwarf virus were added in an LIR-SIR-LIR orientation.
• Vector type: plant T-DNA plasmid
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: GUS
• Alt name: beta-glucuronidase
• Insert Size (bp): 1900
• Promoter: virion-sense LIR and 2x35S
• Tags / Fusion Proteins:
  • NLS (N terminal on insert)
  • flag (N terminal on insert)

Cloning Information for Gene/Insert 1

• Cloning method: Gateway Cloning
• 5′ sequencing primer: aacccgtgaaatcaaaaaact
• 3′ sequencing primer: tcattgtttgcctccctgctg

Gene/Insert 2

• Gene/Insert name: 3kb filler sequence
• Alt name: ALS repair template
• Insert Size (bp): 3000

Cloning Information for Gene/Insert 2

• Cloning method: Gateway Cloning
• 5′ sequencing primer: tgcgagatcgggccggcctggcc
• 3′ sequencing primer: gatattttgaattaaagataa

Resource Information

• A portion of this plasmid was derived from a plasmid made by: GUS was amplified by PCR from a plasmid containing GUS.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Note that there may be some minor discrepancies between Addgene's quality control sequence and the depositor's assembled sequence. These discrepancies are not in functionally relevant regions of the plasmid.