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Addgene

RFP-KIF3A-HL
(Plasmid #51397)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 51397 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    RFP-C1
  • Backbone size w/o insert (bp) 4666
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL10 Gold
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    KIF3A
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1000
  • Entrez Gene
    Kif3a (a.k.a. Kif3, Kifl, Kns3)
  • Promoter CMV
  • Tag / Fusion Protein
    • RFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer None
  • 3′ sequencing primer None
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    RFP-KIF3A-HL was a gift from Trina Schroer (Addgene plasmid # 51397 ; http://n2t.net/addgene:51397 ; RRID:Addgene_51397)

  • For your References section:

    Kinesin-2 is a motor for late endosomes and lysosomes. Brown CL, Maier KC, Stauber T, Ginkel LM, Wordeman L, Vernos I, Schroer TA. Traffic. 2005 Dec;6(12):1114-24. 10.1111/j.1600-0854.2005.00347.x PubMed 16262723
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