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PurposeE. coli expression vector for E. coli RtcB RNA ligase
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 51282 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET-53-DEST
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Backbone manufacturerEMD / Novagen
- Backbone size w/o insert (bp) 5230
- Total vector size (bp) 6430
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameE. coli RtcB
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SpeciesE. coli
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Insert Size (bp)1199
- Promoter T7 RNA Polymerase
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Tag
/ Fusion Protein
- 6xHis (N terminal on backbone)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer T7 promoter
- 3′ sequencing primer T7 terminator (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Use expression strain: BL21 (DE3) RIPL
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET-53-RtcB was a gift from Jay Hesselberth (Addgene plasmid # 51282 ; http://n2t.net/addgene:51282 ; RRID:Addgene_51282) -
For your References section:
Global analysis of RNA cleavage by 5'-hydroxyl RNA sequencing. Peach SE, York K, Hesselberth JR. Nucleic Acids Res. 2015 May 22. pii: gkv536. 10.1093/nar/gkv536 PubMed 26001965