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Addgene

pET21a-Orf2
(Plasmid #51191)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 51191 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET21a
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Orf2
  • Species
    Streptomyces griseus
  • Insert Size (bp)
    870
  • Promoter T7
  • Tag / Fusion Protein
    • 6x-his (N terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

UCSF Case No. SF11-016-2; U.S. Patent Application 13/489,205. GeneArt inserted the synthesized fragment into a pET21a vector.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET21a-Orf2 was a gift from Christopher Voigt (Addgene plasmid # 51191 ; http://n2t.net/addgene:51191 ; RRID:Addgene_51191)

  • For your References section:

    Genomic mining of prokaryotic repressors for orthogonal logic gates. Stanton BC, Nielsen AA, Tamsir A, Clancy K, Peterson T, Voigt CA. Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411. 10.1038/nchembio.1411 PubMed 24316737
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