pGL3-U6-sgRNA-PGK-puromycin
(Plasmid #51133)

• Purpose: (Empty Backbone) expresses sgRNA from the U6 promoter.
• Depositing Lab: Xingxu Huang
• Publication: Shen et al Nat Methods. 2014 Mar 2. doi: 10.1038/nmeth.2857.

Backbone

• Vector backbone: pGL3-Basic
• Backbone manufacturer: Promega
• Vector type: Mammalian Expression, CRISPR
• Promoter: U6
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: pLKO.inducible
• 3′ sequencing primer: O.PGK1b-R

Resource Information

• Supplemental Documents:
  • pGL3-U6-sgRNA-PGK-puromycin
• Articles Citing this Plasmid:
  • 86 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Information for Cloning Grade DNA (Catalog # 51133-DNA.cg) ( Back to top)

Purpose

Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.

Delivery

• Amount: 2 µg
• Guaranteed Concentration: 100 ng/µl +/- 5 ng/µl
• Pricing: $105 USD
• Storage: DNA can be stored at 4℃ (short term) or -20℃ (long term).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Addgene has verified this plasmid using Next Generation Sequencing. Results are available here