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Addgene

pST1374-N-NLS-flag-linker-Cas9-D10A
(Plasmid #51130)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 51130 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Addgene 44758
  • Total vector size (bp) 9318
  • Vector type
    Mammalian Expression, CRISPR
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cas9 nickase
  • Alt name
    Cas9 D10A
  • Insert Size (bp)
    4263
  • Mutation
    D10A (nickase mutant)
  • Promoter CMV
  • Tags / Fusion Proteins
    • NLS (N terminal on insert)
    • Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site ApaI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer bGH-pA-rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pST1374-N-NLS-flag-linker-Cas9-D10A was a gift from Xingxu Huang (Addgene plasmid # 51130 ; http://n2t.net/addgene:51130 ; RRID:Addgene_51130)

  • For your References section:

    Efficient genome modification by CRISPR-Cas9 nickase with minimal off-target effects. Shen B, Zhang W, Zhang J, Zhou J, Wang J, Chen L, Wang L, Hodgkins A, Iyer V, Huang X, Skarnes WC. Nat Methods. 2014 Mar 2. doi: 10.1038/nmeth.2857. 10.1038/nmeth.2857 PubMed 24584192
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