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Addgene

pLenti.PGK.chFP.W
(Plasmid #51008)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 51008 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRRLsin.cPPT.PGKp.eGFP.W
  • Backbone manufacturer
    L Naldini
  • Total vector size (bp) 7375
  • Modifications to backbone
    pLenti.pgk:chFP lentivector derives from the pRRLsin.cPPT.PGKp.eGFP.W lentivector that contains the constitutively expressed human phosphoglycerate kinase promoter (De Palma et al., 2003).
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    mChFP
  • Alt name
    mCherry FP
  • Insert Size (bp)
    711
  • Promoter human PGK

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH1 (not destroyed)
  • 3′ cloning site Sal11 (not destroyed)
  • 5′ sequencing primer T3
  • 3′ sequencing primer SV40pA-R
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti.PGK.chFP.W was a gift from Rusty Lansford (Addgene plasmid # 51008 ; http://n2t.net/addgene:51008 ; RRID:Addgene_51008)
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