pLenti.CAG.H2B-Dendra2.W
(Plasmid #51005)

• Purpose: Lentivector that expresses H2B-Dendra2 from internal CAG enhancer-promoter
• Depositing Lab: Rusty Lansford
• Publication: Lentivirus (unpublished)

Backbone

• Vector backbone: pLenti.CAG
• Total vector size (bp): 9055
• Modifications to backbone: Cut pLenti.CAG.GFP with SalI, blunt, then BamHI. Ligated pLenti.CAG (BamHI/SalI blunt) with H2B-Dendra2 insert (BglII, EcoRV)
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: H2B-Dendra2
• Species: Synthetic
• Insert Size (bp): 1151
• Promoter: CAG
• Tag / Fusion Protein:
  • histone2B (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Sal1 (destroyed during cloning)
• 3′ cloning site: BamH1 (destroyed during cloning)
• 5′ sequencing primer: T3
• 3′ sequencing primer: SV40pA-R

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that the H2B sequence in this plasmid was derived from H2B-GFP (Addgene plasmid# 11680) and contains the same differences (D26G and V119I) compared to GenBank reference sequences, such as NP_066402.2.