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PurposeConstitutive dCas9-KRAB 2nd generation lentiviral expression vector
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Depositing Labs
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 50919 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepHAGE
- Total vector size (bp) 12860
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Vector typeMammalian Expression, Lentiviral, CRISPR
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namedCas9
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SpeciesS. pyogenes
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Insert Size (bp)4460
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MutationD10A, H840A
- Promoter EF1alpha
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Tags
/ Fusion Proteins
- 3xHA (C terminal on insert)
- KRAB (C terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer Ef1alpha-F 5'-TCAAGCCTCAGACAGTGGTTC-3'
- 3′ sequencing primer WPRE-R 5'-CATAGCGTAAAAGGAGCAACA-3' (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHAGE EF1α dCas9-KRAB was a gift from Rene Maehr & Scot Wolfe (Addgene plasmid # 50919 ; http://n2t.net/addgene:50919 ; RRID:Addgene_50919) -
For your References section:
Cas9 effector-mediated regulation of transcription and differentiation in human pluripotent stem cells. Kearns NA, Genga RM, Enuameh MS, Garber M, Wolfe SA, Maehr R. Development. 2014 Jan;141(1):219-23. doi: 10.1242/dev.103341. 10.1242/dev.103341 PubMed 24346702
Map uploaded by Addgene staff.
