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Addgene

pHR SFFVp YFP-PIF-SOS2cat
(Plasmid #50851)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 50851 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHR
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    SOS2cat
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1457
  • Mutation
    residues 566–1049
  • Entrez Gene
    SOS2 (a.k.a. NS9, SOS-2)

Gene/Insert 2

  • Gene/Insert name
    PIF6 1-100
  • Species
    A. thaliana (mustard weed)
  • Insert Size (bp)
    300
  • Tag / Fusion Protein
    • YFP (N terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The discrepancies between the full sequence and Addgene QC sequence, including those in YFP do not affect function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHR SFFVp YFP-PIF-SOS2cat was a gift from Wendell Lim (Addgene plasmid # 50851 ; http://n2t.net/addgene:50851 ; RRID:Addgene_50851)

  • For your References section:

    Using optogenetics to interrogate the dynamic control of signal transmission by the ras/erk module. Toettcher JE, Weiner OD, Lim WA. Cell. 2013 Dec 5;155(6):1422-34. doi: 10.1016/j.cell.2013.11.004. 10.1016/j.cell.2013.11.004 PubMed 24315106
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