-
PurposeFluorescent reporter for membrane PIP3 levels
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 50837 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepHR
-
Vector typeLentiviral, Synthetic Biology
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert namePHAkt
-
Alt namePH domain of Akt
-
Insert Size (bp)491
-
Entrez GeneAKT1 (a.k.a. AKT, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA)
-
Tag
/ Fusion Protein
- Cerulean (C terminal on insert)
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pHR SFFVp PHAkt Cerulean was a gift from Wendell Lim (Addgene plasmid # 50837 ; http://n2t.net/addgene:50837 ; RRID:Addgene_50837) -
For your References section:
Light-based feedback for controlling intracellular signaling dynamics. Toettcher JE, Gong D, Lim WA, Weiner OD. Nat Methods. 2011 Sep 11;8(10):837-9. doi: 10.1038/nmeth.1700. 10.1038/nmeth.1700 PubMed 21909100