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PurposeFluorescent reporter for Erk nuclear translocation
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 50848 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepHR
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameErk2
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SpeciesM. musculus (mouse)
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Entrez GeneMapk1 (a.k.a. 9030612K14Rik, ERK, Erk2, MAPK2, PRKM2, Prkm1, p41mapk, p42mapk)
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Tag
/ Fusion Protein
- TagBFP (N terminal on insert)
Resource Information
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Supplemental Documents
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHR SFFVp BFP-Erk2 was a gift from Wendell Lim (Addgene plasmid # 50848 ; http://n2t.net/addgene:50848 ; RRID:Addgene_50848) -
For your References section:
Using optogenetics to interrogate the dynamic control of signal transmission by the ras/erk module. Toettcher JE, Weiner OD, Lim WA. Cell. 2013 Dec 5;155(6):1422-34. doi: 10.1016/j.cell.2013.11.004. 10.1016/j.cell.2013.11.004 PubMed 24315106