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Addgene

pHR iSH-YFP-PIF
(Plasmid #50841)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 50841 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pHR
  • Vector type
    Lentiviral, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    p85 iSH2
  • Alt name
    PI3K p85 subunit
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    593

Gene/Insert 2

  • Gene/Insert name
    PIF6 1-100
  • Species
    A. thaliana (mustard weed)
  • Insert Size (bp)
    300

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The discrepancies between the full sequence and Addgene QC sequence for YFP do not affect function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHR iSH-YFP-PIF was a gift from Wendell Lim (Addgene plasmid # 50841 ; http://n2t.net/addgene:50841 ; RRID:Addgene_50841)
  • For your References section:

    Light-based feedback for controlling intracellular signaling dynamics. Toettcher JE, Gong D, Lim WA, Weiner OD. Nat Methods. 2011 Sep 11;8(10):837-9. doi: 10.1038/nmeth.1700. 10.1038/nmeth.1700 PubMed 21909100