pHR SFFVp Phy-mCherry-CAAX
(Plasmid #50839)

• Purpose: Optogenetic PI3K activation system; membrane component
• Depositing Lab: Wendell Lim
• Publication: Toettcher et al Nat Methods. 2011 Sep 11;8(10):837-9. doi: 10.1038/nmeth.1700.

Backbone

• Vector backbone: pHR
• Vector type: Lentiviral, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: PhyB 1-908
• Species: A. thaliana (mustard weed)
• Insert Size (bp): 2724
• Promoter: SFFV
• Tag / Fusion Protein:
  • mCherry (C terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: n/a

Resource Information

• Supplemental Documents:
  • Plasmid map (gb format)
• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note this plasmid is a crucial component of the system described in this additional reference: Toettcher JE, Weiner OD, Lim WA, Cell. 2013 Dec 5;155(6):1422-34. doi: 10.1016/j.cell.2013.11.004. (PMID 24315106)