pCAG-EGxxFP
(Plasmid #50716)

• Purpose: 5’ and 3’ EGFP fragments that shares 482bp were placed under ubiquitous CAG promoter. Used for validation of gRNA sequences by DSB mediated EGFP reconstitution.
• Depositing Lab: Masahito Ikawa
• Publication: Mashiko et al Sci Rep. 2013 Nov 27;3:3355. doi: 10.1038/srep03355.

Backbone

• Vector backbone: pCAGGS
• Backbone size w/o insert (bp): 5138
• Total vector size (bp): 6383
• Vector type: Mammalian Expression, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: split EGFP
• Species: Synthetic
• Insert Size (bp): 1239
• Promoter: CAG

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: GCCTTCTTCT TTTTCCTACA GC
• 3′ sequencing primer: GCCACACCAG CCACCACCTT CTG

Resource Information

• Supplemental Documents:
  • pCAG-EGxxFP
• A portion of this plasmid was derived from a plasmid made by: pCAGGS is from Dr. Junichi Miyazaki, original inventor. EGFP was from clontech.
• Articles Citing this Plasmid:
  • 57 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry