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Purposeexpression of Sarm1 in mammalian cells
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 50707 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGW1-CMV-Myc
- Backbone size w/o insert (bp) 5371
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Modifications to backboneMyc tag added to pGW1-CMV
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSarm1
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Alt namesterile alpha and HEAT/Armadillo motif containing 1
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SpeciesM. musculus (mouse)
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Insert Size (bp)2172
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GenBank IDNM_172795
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Entrez GeneSarm1 (a.k.a. A830091I15Rik, C78606, MyD885, Sarm)
- Promoter CMV
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Tag
/ Fusion Protein
- Myc (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BglII (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer ctgagcagtactcgttgc (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The mouse Sarm1 IMAGE clone was ordered from Invitrogen and further subcloned into the vector pGW1-CMV with a Myc tag. The final product pGW1-Myc-Sarm1 is going to deposit to Addgene.
There is a 1bp insertion in Addgene's quality control sequence when compared to the depositor's reference sequence. The insertion does not affect epitope tag or ORF.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGW1-Myc-Sarm1 was a gift from Yi-Ping Hsueh (Addgene plasmid # 50707 ; http://n2t.net/addgene:50707 ; RRID:Addgene_50707) -
For your References section:
Sarm1, a negative regulator of innate immunity, interacts with syndecan-2 and regulates neuronal morphology. Chen CY, Lin CW, Chang CY, Jiang ST, Hsueh YP. J Cell Biol. 2011 May 16;193(4):769-84. doi: 10.1083/jcb.201008050. Epub 2011 May 9. 10.1083/jcb.201008050 PubMed 21555464
Map uploaded by the depositor.
![](https://media.addgene.org/data/easy-thumbnails/data/26/28/fe259544-60da-11e3-bedc-000c29055998.pdf.940x940_q85_autocrop.png)