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Addgene

pCph8
(Plasmid #50552)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 50552 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pProTet.E333
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 1995
  • Total vector size (bp) 4231
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cph8
  • Species
    Synthetic
  • Insert Size (bp)
    4230

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Encodes the Cph8 (Cph1/EnvZ fusion) light sensor kinase/phosphatase downstream of the pLTetO-1 promoter.
Requires pPLPCB(S)t or some other source of TetR
Requires PCB to function
Does NOT include the response regulator.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCph8 was a gift from Jeffrey Tabor (Addgene plasmid # 50552 ; http://n2t.net/addgene:50552 ; RRID:Addgene_50552)

  • For your References section:

    Characterizing bacterial gene circuit dynamics with optically programmed gene expression signals. Olson EJ, Hartsough LA, Landry BP, Shroff R, Tabor JJ. Nat Methods. 2014 Mar 9. doi: 10.1038/nmeth.2884. 10.1038/nmeth.2884 PubMed 24608181
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