pTRE-Tight-MitoTimer
(Plasmid #50547)

• Purpose: Expresses the Timer protein targeted to the mitochondria under a Tet-inducible promoter
• Depositing Lab: Roberta Gottlieb
• Publication: Hernandez et al Autophagy. 2013 Nov 1;9(11):1852-61. doi: 10.4161/auto.26501. Epub 2013 Oct 11.

Backbone

• Vector backbone: pTRE Tight
• Backbone manufacturer: Clonetech
• Backbone size w/o insert (bp): 2516
• Total vector size (bp): 3325
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MitoTimer
• Insert Size (bp): 809
• Mutation: F12S mutation in Timer

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: AGG CGT ATC ACG AGG CCC TTT CGT
• 3′ sequencing primer: TAT TAC CGC CTT TGA GTG AGC TGA

Resource Information

• Articles Citing this Plasmid:
  • 11 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The F12S mutation in Timer does not affect the function of the plasmid, as described in the associated publication.

The Timer sequence is preceded by the mitochondrial targeting sequence from cytochrome c oxidase subunit 8A: MSVLTPLLLRGLTGSARRLPVPRAKIHSL