hCEP120-EGFP
(Plasmid #50382)

• Purpose: Over-expression of EGFP-tagged hCEP120
• Depositing Lab: Tang Tang
• Publication: Lin et al J Cell Biol. 2013 Jul 22;202(2):211-9. doi: 10.1083/jcb.201212060. Epub 2013 Jul 15.

Backbone

• Vector backbone: pEGFP-N2
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4737
• Total vector size (bp): 7705
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Human CEP120
• Alt name: CCDC100
• Species: H. sapiens (human)
• Insert Size (bp): 2962
• GenBank ID: NM_153223
• Entrez Gene: CEP120 (a.k.a. CCDC100)
• Promoter: CMV
• Tag / Fusion Protein:
  • EGFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: CMV forward
• 3′ sequencing primer: EGFP-N

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

There are 4 different nucleotides in our clone comparing to NM_153223. These nucleotides could all be found in dbSNP (rs6876883, rs6595440, rs1047437, rs1047438). Sequence of the entire ORF is available for download.