pLYS5-SDHB-Flag
(Plasmid #50055)

• Purpose: Expression of human SDHB in mammalian cells
• Depositing Lab: Vamsi Mootha
• Publication: Sancak et al Science. 2013 Nov 14.

Backbone

• Vector backbone: pLYS5
• Total vector size (bp): 8902
• Modifications to backbone: Flag tag C-terminal
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL10 Gold
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: SDHB
• Species: H. sapiens (human)
• Insert Size (bp): 831
• GenBank ID: NM_003000
• Entrez Gene: SDHB (a.k.a. CWS2, IP, MC2DN4, PGL4, PPGL4, SDH, SDH1, SDH2, SDHIP)
• Tag / Fusion Protein:
  • Flag (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Nhe1 (not destroyed)
• 3′ cloning site: Xma1 (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: custom

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry