pDEST-mCherry-GJA1-20k-N1
(Plasmid #49861)

• Purpose: Encodes C-terminal fragment of human connexin 43 initiating at M213 with a V5 C-terminal fusion tag
• Depositing Lab: Robin Shaw
• Publication: Smyth et al Cell Rep. 2013 Nov 5. pii: S2211-1247(13)00578-0. doi: 10.1016/j.celrep.2013.10.009.

Backbone

• Vector backbone: pmcherry-N1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4722
• Total vector size (bp): 5232
• Modifications to backbone: Gateway reading frame cassette c.1 inserted between Afe I and Sma I sites of MCS.
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GJA1-20k
• Alt name: GJA1
• Alt name: Connexin 43
• Species: H. sapiens (human)
• Insert Size (bp): 510
• Mutation: GJA1-20k corresponds to residues 213 to 382 to Connexin 43. No stop codon to create c-terminal fusion
• GenBank ID: NM_000165.3
• Entrez Gene: GJA1 (a.k.a. AVSD3, CMDR, CX43, EKVP, EKVP3, GJAL, HLHS1, HSS, ODDD, PPKCA)
• Promoter: CMV
• Tag / Fusion Protein:
  • mCherry (C terminal on backbone)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: CMV
• 3′ sequencing primer: mCherrry

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry