pOG2002
(Plasmid #49514)

• Purpose: (Empty Backbone) TALEN backbone for expression of TALE(deltaRDV)-FokI AS in C. elegans
• Depositing Lab: Guangshuo Ou
• Publication: Cheng et al Nat Biotechnol. 2013 Oct;31(10):934-7. doi: 10.1038/nbt.2674. Epub 2013 Aug 18.

Backbone

• Vector backbone: pPD95.77
• Backbone manufacturer: Andrew Fire lab
• Vector type: Worm Expression
• Promoter: hsp-16.2
• Tags / Fusion Proteins:
  • SV40 NLS (N terminal on backbone)
  • 3xFLAG (N terminal on backbone)
  • FokI-AS (C terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: M13-F20

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

725 bp of unc54 3'UTR was used for C. elegans expression.
mutations in pthA: delta 1-152, delta 299-875, delta 949-1163
mutations in ZNF-fokI: E484A and R487S

This vector and Addgene plasmid # 49516 are used for insertion of custom repeats which can be assembled by the unit assembly method.