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Addgene

pRF-TetR
(Plasmid #49374)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 49374 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pEXT20
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    TetR repressor
  • Species
    Tn10

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Plasmid also contains YFP reporter (whose expression is controlled by a constitutive promoter containing an operator site for the indicated repressor).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRF-TetR was a gift from Christopher Voigt (Addgene plasmid # 49374 ; http://n2t.net/addgene:49374 ; RRID:Addgene_49374)
  • For your References section:

    Genomic mining of prokaryotic repressors for orthogonal logic gates. Stanton BC, Nielsen AA, Tamsir A, Clancy K, Peterson T, Voigt CA. Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411. 10.1038/nchembio.1411 PubMed 24316737