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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 49372 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepEXT20
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Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH10B
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameSrpR repressor
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SpeciesPseudomonas putida
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer NA
- 3′ sequencing primer EGFP-N (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Plasmid also contains YFP reporter (whose expression is controlled by a constitutive promoter containing an operator site for the indicated repressor).
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRF-SrpR was a gift from Christopher Voigt (Addgene plasmid # 49372 ; http://n2t.net/addgene:49372 ; RRID:Addgene_49372) -
For your References section:
Genomic mining of prokaryotic repressors for orthogonal logic gates. Stanton BC, Nielsen AA, Tamsir A, Clancy K, Peterson T, Voigt CA. Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411. 10.1038/nchembio.1411 PubMed 24316737
