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Addgene

pMPRA3
(Plasmid #49351)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 49351 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pGL4
  • Backbone manufacturer
    Promega
  • Backbone size (bp) 5802
  • Modifications to backbone
    Removed luc2 gene. Inserted cat-ccdB and SV40-H2Kk cassettes.
  • Vector type
    Mammalian Expression
  • Selectable markers
    Truncated H2Kk cell surface receptor

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin, 25 & 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    High Copy

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Promega and Genscript (synthetic SV40-H2Kk insert)
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene sequencing detected an IS1 transposable element

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMPRA3 was a gift from Tarjei Mikkelsen (Addgene plasmid # 49351)

  • For your References section:

    Massively parallel reporter assays in cultured Mammalian cells. Melnikov A, Zhang X, Rogov P, Wang L, Mikkelsen TS. J Vis Exp. 2014 Aug 17;(90). doi: 10.3791/51719. 10.3791/51719 PubMed 25177895
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