pJFA334E9
(Plasmid #49237)

• Purpose: TET1-catalytic domain (CD) expression vector for TALE-TET1 fusion protein to induce targeted demethylation in mammalian cells
• Depositing Lab: Keith Joung
• Publication: Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038/nbt.2726.

Backbone

• Vector backbone: unknown
• Backbone manufacturer: Joung lab
• Vector type: TALEN ; TALE-TET1 Expression Vector

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: TET1 tet methylcytosine dioxygenase 1
• Species: H. sapiens (human), Synthetic
• Entrez Gene: TET1 (a.k.a. CXXC6, LCX, bA119F7.1)
• Promoter: EF1a

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: OMM520
• 3′ sequencing primer: OJA199

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Location of plasmid features:
EF1alpha promoter = 922-2106
NLS = 2118-2142
TALE N-terminal domain = 2167-2577
Clone TALE array repeats between BsmBI sites
TALE C-terminal domain = 2665-2949
TET1 catalytic domain = 2956-5123
BGH polyA = 6369-6596