pBSFI-myr-akt-T308A
(Plasmid #49189)

• Purpose: cDNA of Akt1 with myristyolation signal and point mutation from T to A at position 308 in cloning vector
• Depositing Lab: Peter Vogt
• Publication: Aoki et al Proc Natl Acad Sci U S A. 1998 Dec 8;95(25):14950-5.

Backbone

• Vector backbone: pBSFI
• Backbone size w/o insert (bp): 3000
• Total vector size (bp): 4500
• Modifications to backbone: pBSFI is based on pBluescript SK, with a different MCS inserted between SacI and KpnI
• Vector type: Adaptor Plasmid

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Akt1
• Alt name: Akt
• Species: M. musculus (mouse)
• Insert Size (bp): 1500
• Mutation: T308A
• GenBank ID: NM_009652.3
• Entrez Gene: Akt1 (a.k.a. Akt, LTR-akt, PKB, PKB/Akt, PKBalpha, Rac)
• Promoter: T7
• Tags / Fusion Proteins:
  • myristolation signal (N terminal on insert)
  • HA tag (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (destroyed during cloning)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: T3
• 3′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry