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Addgene

pFBXNH3
(Plasmid #49049)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 49049 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFBDM
  • Backbone manufacturer
    Imre Berger
  • Backbone size (bp) 6986
  • Modifications to backbone
    modified for compatibility with FX cloning and added tags/fusion proteins
  • Vector type
    Insect Expression
  • Promoter polH (polyhedrin)
  • Tags / Fusion Proteins
    • decaHis (10x His) (N terminal on backbone)
    • 3C protease site (PreScission site) (N terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin, 25 & 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DB3.1
  • Copy number
    Low Copy

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Constructed by Christopher Weinert, Grütter lab, Dept of Biochemistry, University of Zurich, Switzerland.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFBXNH3 was a gift from Markus Gruetter (Addgene plasmid # 49049 ; http://n2t.net/addgene:49049 ; RRID:Addgene_49049)

  • For your References section:

    Crystal structure of TRIM20 C-terminal coiled-coil/B30.2 fragment: implications for the recognition of higher order oligomers. Weinert C, Morger D, Djekic A, Grutter MG, Mittl PR. Sci Rep. 2015 Jun 4;5:10819. doi: 10.1038/srep10819. 10.1038/srep10819 PubMed 26043233
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