pCMV_dCas9_VP64
(Plasmid #49015)

• Purpose: encodes human-optimized dCas9_VP64 synthetic transcription factor
• Depositing Lab: Timothy Lu
• Publication: Farzadfard et al ACS Synth Biol. 2013 Sep 11.

Backbone

• Vector backbone: phi-Yellow-Dest
• Backbone manufacturer: Evrogen
• Backbone size w/o insert (bp): 4000
• Total vector size (bp): 9760
• Modifications to backbone: Inserted dCas9-3xNLS-VP64 along with pPGK1_mKATE-HSVpolyA
• Vector type: Mammalian Expression, CRISPR, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: dCas9_VP64 (human-codon-optimized)
• Species: H. sapiens (human)
• Insert Size (bp): 4410
• Promoter: CMV
• Tag / Fusion Protein:
  • SV40 NLS (C terminal on insert)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: TCTTCGCTATTACGCCAGCCC
• 3′ sequencing primer: AGTGCGGCGACGATAGTCATGCCCC

Resource Information

• Supplemental Documents:
  • Genbank file
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry

Depositor Comments

L591M mutation with dCas9