645 pCS2-etv2
(Plasmid
#49005)
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PurposeMammalian Expression vector, In-situ probe template and in vitro transcription vector for zebrafish Etv2
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 49005 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCS-dest
- Total vector size (bp) 5259
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Vector typeMammalian Expression ; invitro transcription, riboprobe template
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameets variant gene 2
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Alt nameetv2
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SpeciesD. rerio (zebrafish)
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Insert Size (bp)1133
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Entrez Geneetsrp (a.k.a. ets1b, etv2, grc, wu:fc49b07, zgc:153819)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer Sp6
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
645 pCS2-etv2 was a gift from Nathan Lawson (Addgene plasmid # 49005 ; http://n2t.net/addgene:49005 ; RRID:Addgene_49005) -
For your References section:
Post-transcriptional mechanisms contribute to Etv2 repression during vascular development. Moore JC, Sheppard S, Shestopalov IA, Chen JK, Lawson N. Dev Biol. 2013 Sep 11. pii: S0012-1606(13)00470-3. doi: 10.1016/j.ydbio.2013.08.028. 10.1016/j.ydbio.2013.08.028 PubMed 24036310
