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PurposeProvides alcR as GreenGate coding sequence module.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 48825 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUC19
- Backbone size w/o insert (bp) 2686
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Vector typeGolden Gate compatible cloning vector
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namealcR
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SpeciesSynthetic
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Insert Size (bp)2465
Cloning Information
- Cloning method Unknown
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This plasmid is designed for Golden Gate cloning using the BsaI enzyme.
Plasmid Features (listed as bp in full plasmid sequence):
BsaI site #1 = 2241-2251bp
alcR coding sequence = 2252-4716bp
BsaI site #2 = 4717-4727bp
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGGC011 was a gift from Jan Lohmann (Addgene plasmid # 48825 ; http://n2t.net/addgene:48825 ; RRID:Addgene_48825) -
For your References section:
GreenGate - A Novel, Versatile, and Efficient Cloning System for Plant Transgenesis. Lampropoulos A, Sutikovic Z, Wenzl C, Maegele I, Lohmann JU, Forner J. PLoS One. 2013 Dec 20;8(12):e83043. doi: 10.1371/journal.pone.0083043. PubMed 24376629