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PurposeCAG promoter driving Cre-ERT2 fusion-IRES-Blasticidin resistance
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 48760 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneBluescript
- Backbone size w/o insert (bp) 6100
- Total vector size (bp) 8135
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Vector typeMammalian Expression ; pPy
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCre::ERT2
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SpeciesSynthetic
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Insert Size (bp)2000
- Promoter CAG
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Tag
/ Fusion Protein
- ERT2 (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer CAG forward
- 3′ sequencing primer pA backward (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPy-CAG-Cre::ERT2-IRES-BSD was a gift from Austin Smith (Addgene plasmid # 48760 ; http://n2t.net/addgene:48760 ; RRID:Addgene_48760) -
For your References section:
Exit from pluripotency is gated by intracellular redistribution of the bHLH transcription factor Tfe3. Betschinger J, Nichols J, Dietmann S, Corrin PD, Paddison PJ, Smith A. Cell. 2013 Apr 11;153(2):335-47. doi: 10.1016/j.cell.2013.03.012. 10.1016/j.cell.2013.03.012 PubMed 23582324
Map uploaded by the depositor.
