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Addgene

pCMV10-Flag-Fbxl21
(Plasmid #48752)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 48752 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    p3xFLAG-CMV-10
  • Backbone manufacturer
    Sigma
  • Backbone size w/o insert (bp) 6299
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Fbxl21
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Fbxl21 (a.k.a. D630045D17Rik, FBL3B, FBXL3B, Psttm)
  • Tag / Fusion Protein
    • 3XFLAG (N terminal on backbone)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV10-Flag-Fbxl21 was a gift from Joseph Takahashi (Addgene plasmid # 48752 ; http://n2t.net/addgene:48752 ; RRID:Addgene_48752)

  • For your References section:

    Competing E3 ubiquitin ligases govern circadian periodicity by degradation of CRY in nucleus and cytoplasm. Yoo SH, Mohawk JA, Siepka SM, Shan Y, Huh SK, Hong HK, Kornblum I, Kumar V, Koike N, Xu M, Nussbaum J, Liu X, Chen Z, Chen ZJ, Green CB, Takahashi JS. Cell. 2013 Feb 28;152(5):1091-105. doi: 10.1016/j.cell.2013.01.055. 10.1016/j.cell.2013.01.055 PubMed 23452855
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