pGL3 Pro Mut E2
(Plasmid #48750)

• Purpose: Expression of luciferase driven by mPer2 promoter fragment containing mutated E-box2 (bases -112 to +98 with respect to transcription start site at +1) and SV40 promoter (backbone)
• Depositing Lab: Joseph Takahashi
• Publication: Yoo et al Proc Natl Acad Sci U S A. 2005 Feb 15;102(7):2608-13. Epub 2005 Feb 7.

Backbone

• Vector backbone: pGL3-Promoter
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 5010
• Vector type: Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: mPer2 promoter/enhancer region containing mutated E-box2 (-112 to +98)
• Species: M. musculus (mouse)
• Mutation: Mutated E-box2 sequence from CACGTT to GCTAGT

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Minor sequencing discrepancies may exist between the mPer2 promoter reference sequence and Addgene's sequencing results, but do not affect the function of the plasmid.