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Addgene

SNF-5 pXOOM
(Plasmid #48697)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 48697 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pXOOM
  • Backbone size w/o insert (bp) 5052
  • Total vector size (bp) 7239
  • Vector type
    Mammalian Expression ; Xenopus Oocyte Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    Room Temperature
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    snf-5
  • Alt name
    CeNAT5
  • Species
    C. elegans (nematode)
  • Mutation
    K42E (when compared to NP_496735.1)
  • Entrez Gene
    snf-5 (a.k.a. CELE_Y46G5A.30)
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer ATG GCC GAT TCG GGC AGC AAT GA
  • 3′ sequencing primer T TAA TAA ACT TGA TGA TAT GTA
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SNF-5 pXOOM was a gift from Dmitri Boudko (Addgene plasmid # 48697 ; http://n2t.net/addgene:48697 ; RRID:Addgene_48697)

  • For your References section:

    An SLC6 transporter of the novel B(0,)- system aids in absorption and detection of nutrient amino acids in Caenorhabditis elegans. Metzler R, Meleshkevitch EA, Fox J, Kim H, Boudko DY. J Exp Biol. 2013 Aug 1;216(Pt 15):2843-57. doi: 10.1242/jeb.081497. Epub 2013 Apr 11. 10.1242/jeb.081497 PubMed 23580723
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