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Addgene

Oct4-IRES-eGFP-PGK-Neo
(Plasmid #48681)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 48681 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCR2.1TOPO
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Oct4
  • Alt name
    Pou5f1
  • Alt name
    Oct3
  • Alt name
    Otf3
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    3000
  • GenBank ID
    NC_000083.6
  • Entrez Gene
    Pou5f1 (a.k.a. NF-A3, Oct-3, Oct-3/4, Oct-4, Oct3, Oct3/4, Oct4, Otf-3, Otf-4, Otf3, Otf3-rs7, Otf3g, Otf4)
  • Tag / Fusion Protein
    • GFP (C terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Oct4-IRES-eGFP-PGK-Neo was a gift from Rudolf Jaenisch (Addgene plasmid # 48681 ; http://n2t.net/addgene:48681 ; RRID:Addgene_48681)

  • For your References section:

    One-Step Generation of Mice Carrying Reporter and Conditional Alleles by CRISPR/Cas-Mediated Genome Engineering. Yang H, Wang H, Shivalila CS, Cheng AW, Shi L, Jaenisch R. Cell. 2013 Aug 27. pii: S0092-8674(13)01016-7. doi: 10.1016/j.cell.2013.08.022. 10.1016/j.cell.2013.08.022 PubMed 23992847
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